More than 95% of the Guadalquivir River-Mid Section Special Area of Conservation is located in the province of Cordoba, as well as in some municipalities of Jaen and Seville. Namely, Lopera, Marmolejo, Adamuz, Almodovar del Río Bujalance, El Carpio, Cordoba, Fuente Palmera, Hornachuelos, Montoro, Palma del Río, Pedro Abad, Posadas, Villa del Río and Villafranca de Córdoba. It occupies an area of around 2,549 hectares and does not include all of the middle section of the Guadalquivir River, but rather, two different parts of it. The first section begins 6 kilometres upstream from Villa del Río, and runs as far as Las Quemadillas in Cordoba capital. The second section begins 200 metres from the Huerta de Valdehermoso Stream, in Alameda del Obispo, and ends 3 kilometres downstream from Peñaflor, leaving an area free at the top of Cordoba capital. The Sotos de la Albolafia Natural Monument is located in this free space, between the two sections that make up the Guadalquivir River-Mid Section SAC.
The east part of the SAC is characterised by riparian vegetation, consisting of poplar groves and some willow trees. Further in from the river, there are elm trees. Downstream, the first band of willow trees disappears, giving way to poplar groves and tamarisk. Further down there are also stretches of black poplars, which appear next to eucalyptus repopulations and other groves of repopulated black, and white poplar. There are also riverine elms from eutrophic waters that, when in good condition, provide dense coverage, and are sometimes accompanied by white poplars. In the bushy rock areas, there are blackberries and lianas such as black bryony, bryony and water-hemlock.
The wildlife is hugely diverse. Inevitably, there are typical riverbank species; numerous shorebirds and herons, as well as birds of prey, many of them threatened species. The Iberian spotted frog deserves special mention as it is an endemic Iberian species, with 90% of its population based in Andalusia. It is also considered a positive indicator of good riverbank conditions.
Characteristic species of river ecosystems include the otter, as well as some amphibians, reptiles, dragonfly species and also a significant amount of fish, with the freshwater blenny, common roach, Cobitis paludica and Iberochondrostoma lemmingii being the most prominent.